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Core Equipments of IMIL

Olympus Fluoview FV3000 Confocal Laser Scanning MicroscopeFV-3000.jpg

  • Olympus IX83 fully motorized inverted microscope with ZDC laser-based autofocus
  • Resonant scanner for high-speed acquisition and galvanometer scanner for high definition
  • Automated microscope stage
  • 4 High-Sensitivity GaAsP detectors
  • Tokai hit stage-top live-cell incubation chamber
  • Transmitted detector for Brightfield/DIC image acquisition
  • Analog and digital in-out box for synchronization with external devices

 

Available Laser Lines (nm): 405-445-488-514-561-640

Objectives

Objective (UV corrected) Mag. NA Immersion WD (μm) DIC Color ID
PlanApo 2x 0.08 dry 6200 No white
U Plan S-Apo 10x 0.4 dry 3000 Yes* yellow
U Plan S-Apo 20x 0.75 dry 600 Yes green
U Plan S-Apo N W/CC 30x 1.05 silicone oil 800 Yes green/red
U Plan S-Apo N W/CC, BFP1 40x 1.25 silicone oil 300 Yes blue/red
PlanApo N SC2, BFP1 60x 1.4 oil 120 Yes dark blue/black
U Apo N 100XTIRF 100x 1.49 oil 100 Yes white/black

Filter cubes for eye viewing only

  • DAPI 395/25X, BS425, 460/50M
  • EGFP/FITC/CY2 470/40X, BS495, 525/50M
  • DSRED/TRITC/Cy3 545/30X, BS570, 620/60M

Olympus VS120 Virtual Slide Scanning SystemOLYMPUS VS120 VIRTUAL SLIDE SCANNING SYSTEM

  • 100-slide capacity for high throughput Imaging
  • Transmitted light brightfield and epi-fluorescence scanning
  • Automated sample identification and focus.
  • Advanced stitching for tiled montage images

Fluorescence light source: LED Lumencor SOLA

Digital monochrome camera: Hamamatsu ORCA Flash4.0 V2

Color camera: 2/3” CCD camera, 3.45 μm x 3.45 μm pixel size

Fluorescence filters:

  • DAPI ZERO LED: Ex392/23, Em447/60, Dichroic409 (DAPI, AF405, BFP, Pacific Blue)
  • FITC ZERO LED: Ex474/27, Em525/45, Dichroic495 (FITC, GFP, Cy2, AF488)
  • MCherry ZERO LED: Ex578/21, Em641/75, Dichroic596 (mCherry, TexasRed, mRFP)
  • Cy5 ZERO LED: Ex635/18, Em680/42, Dichroic652 (Cy5, AF647, APC)

Objectives

Objective Mag. NA Immersion WD (μm)
PlanApo N 2x 0.08 dry 6200
UPlanSApo 10x 0.4 dry 3100
UPlanSApo 20x 0.75 dry 600
UPlanSApo 40x 0.95 dry 180

  

OLYMPUS FLUOVIEW FVMPE-RS Multiphoton LASER SCANNING MICROSCOPEfvmpe_rs_crop.jpg

  • Upright microscope with gantry frame, optimized for intravital imaging with a large space for live animals and experimental equipment
  • Inner-focus articulating nosepiece will image at any angle
  • Dual wavelength laser with a tunable range from 680 nm to 1300 nm and a fixed wavelength line at 1045 nm enables simultaneous multi-color, multiphoton imaging
  • Resonant scanner for high-speed acquisition and galvonometer scanner for high definition
  • 2 High-Sensitivity GaAsP detectors

 

Available Laser Lines (nm): Tunable 680-1300, Fixed 1045

 

Objectives

Objective

Mag.

NA

Immersion

WD (μm)

UMPLFLN 10XW

10X

0.3

Water

3500

XLPLN25XWMP2

25X

1.05

Water

2000

LUMPLFLN40XW

40X

0.8

Water

3300

 

Filter cubes for eye viewing only

  • DAPI 395/25X, BS425, 460/50M
  • EGFP/FITC/CY2 470/40X, BS495, 525/50M
  • DSRED/TRITC/Cy3 545/30X, BS570, 620/60M

Leica SP2 Confocal Laser Scanning MicroscopeLeica SP2 Confocal Laser Scanning Microscope

  • Leica DMIRE-2 motorized inverted microscope
  • AOBS spectral confocal scanning head
  • R9624 Hamamatsu Photonics PMTs

Available Laser Lines (nm): 458-476-488-496-514-543-594-633

Objectives

Objective Mag. NA Immersion UV Working Distance (μm) IC prism Color ID
HC PL APO CS 10x 0.4 dry yes 2200 A Yellow
HC PL APO CS 20x 0.7 dry yes 590 C Green
HCX PL APO CS 40x 1.25 oil yes 100 E Blue
HCX PL APO CS 63x 1.4 oil no 100 E Dark blue
HCX PL APO CS 63x 1.2 water yes 220 - Dark blue
U APO 340/cc 40x 1.15 water yes 260 - Rubber cap

Fluorescence filters for eye viewing only:

  • I3 Cube: Ex BP450-490, Dichroic 510, Em LP 515 (FITC, GFP, Cy2, AF488)
  • N2.3 Cube: Ex BP515-560, Dichroic 580, Em LP 590 (mCherry, TRITC, RFP)

 

NanoFluor II Integrated Microscope SystemNANOFLUOR II INTEGRATED MICROSCOPE

  • IX-81 Olympus inverted microscope with focus stabilizer
  • Yokogawa CSU-22 motorized confocal scanning head
  • Ultra-fast spinning disk confocal with Z-piezo control (up to 100 frames per second)
  • TIRFM (Total Internal Reflection Fluorescence Microscopy) attachment
  • Atomic Force Bioscope SZ closed loop I
  • Ar/Kr gas laser

Available Laser Lines (nm)

  • Spinning Disk:488-514-568-647
  • Ultra-fast Sinning Disk: 488-568

Objectives

Objective Mag. NA Immersion UV Working Distance (μm) Color ID
U PL FLN 10x 0.3 dry no 10000 Yellow
U PL FLN PH 40x 0.75 dry no 510 Light blue
U PL FLN 40x 1.3 oil no 200 Light blue
U PL S APO 100x 1.4 oil no 120 white
PLAPON TIRFM 60x 1.45 oil no 100 Dark blue
PL APO 60x 1 water no 150 Dark blue & white