Core Equipments of IMIL
Olympus Fluoview FV3000 Confocal Laser Scanning Microscope
- Olympus IX83 fully motorized inverted microscope with ZDC laser-based autofocus
- Resonant scanner for high-speed acquisition and galvanometer scanner for high definition
- Automated microscope stage
- 4 High-Sensitivity GaAsP detectors
- Tokai hit stage-top live-cell incubation chamber
- Transmitted detector for Brightfield/DIC image acquisition
- Analog and digital in-out box for synchronization with external devices
Available Laser Lines (nm): 405-445-488-514-561-640
Objectives
| Objective (UV corrected) | Mag. | NA | Immersion | WD (μm) | DIC | Color ID |
|---|---|---|---|---|---|---|
| PlanApo | 2x | 0.08 | dry | 6200 | No | white |
| U Plan S-Apo | 10x | 0.4 | dry | 3000 | Yes* | yellow |
| U Plan S-Apo | 20x | 0.75 | dry | 600 | Yes | green |
| U Plan S-Apo N W/CC | 30x | 1.05 | silicone oil | 800 | Yes | green/red |
| U Plan S-Apo N W/CC, BFP1 | 40x | 1.25 | silicone oil | 300 | Yes | blue/red |
| PlanApo N SC2, BFP1 | 60x | 1.4 | oil | 120 | Yes | dark blue/black |
| U Apo N 100XTIRF | 100x | 1.49 | oil | 100 | Yes | white/black |
Filter cubes for eye viewing only
- DAPI 395/25X, BS425, 460/50M
- EGFP/FITC/CY2 470/40X, BS495, 525/50M
- DSRED/TRITC/Cy3 545/30X, BS570, 620/60M