Core Equipments of IMIL
Olympus VS120 Virtual Slide Scanning System
- 100-slide capacity for high throughput Imaging
- Transmitted light brightfield and epi-fluorescence scanning
- Automated sample identification and focus.
- Advanced stitching for tiled montage images
Fluorescence light source: LED Lumencor SOLA
Digital monochrome camera: Hamamatsu ORCA Flash4.0 V2
Color camera: 2/3” CCD camera, 3.45 μm x 3.45 μm pixel size
Fluorescence filters:
- DAPI ZERO LED: Ex392/23, Em447/60, Dichroic409 (DAPI, AF405, BFP, Pacific Blue)
- FITC ZERO LED: Ex474/27, Em525/45, Dichroic495 (FITC, GFP, Cy2, AF488)
- MCherry ZERO LED: Ex578/21, Em641/75, Dichroic596 (mCherry, TexasRed, mRFP)
- Cy5 ZERO LED: Ex635/18, Em680/42, Dichroic652 (Cy5, AF647, APC)
Objectives
| Objective | Mag. | NA | Immersion | WD (μm) |
|---|---|---|---|---|
| PlanApo N | 2x | 0.08 | dry | 6200 |
| UPlanSApo | 10x | 0.4 | dry | 3100 |
| UPlanSApo | 20x | 0.75 | dry | 600 |
| UPlanSApo | 40x | 0.95 | dry | 180 |