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Core Equipments of IMIL

Leica SP2 Confocal Laser Scanning Microscope

Leica SP2 Confocal Laser Scanning Microscope

  • Leica DMIRE-2 motorized inverted microscope
  • AOBS spectral confocal scanning head
  • R9624 Hamamatsu Photonics PMTs

Available LASER Lines (nm): 458-476-488-496-514-543-594-633

Objectives

Objective Mag. NA Immersion UV Working Distance (μm) DIC prism Color ID
HC PL APO CS 10x 0.4 dry yes 2200 A Yellow
HC PL APO CS 20x 0.7 dry yes 590 C Green
HCX PL APO CS 40x 1.25 oil yes 100 E Blue
HCX PL APO CS 63x 1.4 oil no 100 E Dark blue
HCX PL APO CS 63x 1.2 water yes 220 - Dark blue
U APO 340/cc 40x 1.15 water yes 260 - Rubber cap

Fluorescence filters for eye viewing only:

  • I3 Cube: Ex BP450-490, Dichroic 510, Em LP 515 (FITC, GFP, Cy2, AF488)
  • N2.3 Cube: Ex BP515-560, Dichroic 580, Em LP 590 (mCherry, TRITC, RFP)