JML Equipment

Picoquant Luminosa- Single Photon Counting Confocal Microscope

• Olympus IX73 partially motorized inverted microscope with autofocus
• FLIMbee fast confocal scanner
• Micrometer (xy) positioning sample stage
• 4 single-photon avalanche diode (SPAD) detectors
• Stage-top live-cell incubation chamber (to be ordered)
• Time-correlated single photon counting (TCSPC) event timer
• Epifluorescence and DIC imaging
• Core methodologies:
  • Fluorescence Lifetime Imaging (FLIM)
  • Single-Molecule Fluorescence Energy Transfer (smFRET)
  • FLIM-FRET
  • Fluorescence Correlation Spectroscopy (FCS)
  • Anisotropy Imaging 

Available Laser Lines (all can be run in pulsed and continuous-wave mode): 406, 478, 531, 560, and 638 nm

Objectives

 Instrument Configuration:

Filters (most links identify what we have, though some are close approximations):

Epifluorescence Filter Cube

• EGFP/FITC/CY2 466/40, BS495, 525/50 (AHF Analysentechnik F36-527/Semrock 4050B)

Excitation Dichroics

• Quadband Excitation: Semrock Di03-R405/488/532/635
• Quadband Excitation: Semrock Di01-R405/488/561/635
• Triband Excitation (includes 405 nm): Chroma zt488/640rpc
• Triband Excitation (includes 405 nm): Chroma zt/532/640rpc

Emission Dichroic Splitters (between detectors 1/2 & 3/4)

• Polarization Beam Splitter
• 50/50 Beam Splitter
• Chroma zt532rdc
• Chroma 560lpxr

Emission Splitters (between detectors 1 & 2)

• Mirror
• Chroma T635lpxr

Emission Splitters (between detectors 3 & 4)

• Mirror
• AHF Analysentechnik H488LPXR
• Chroma T635lpxr

Emission Filters (detector 1)

• Semrock 520/35
• Semrock 582/64
• Chroma 595/40

Emission Filters (detector 2)

• AHF Analysentechnik 690/70
• Chroma ET700/75m

Emission Filters (detector 3)

• Chroma ET445/30x
• Semrock 582/64

Emission Filters (detector 4)

• Semrock 511/20
• Semrock 520/35
• AHF Analysentechnik 690/70
• Chroma ET700/75m